P. tremuloides are unique, because they are clonal. They can sexually reproduce, but Colorado has not had the conditions for this to occur in a while. The trees do produce seeds; however, the seeds do not germinate when they are in the ground. The way that P. tremuloides grow is difficult when distinguishing them from one another. Also, there is no way to tell how the individuals are related. With no way to tell, speculations are made to determine how the individuals are related (Figure 2). Sampling P. tremuloides in close proximity increases the chance that the samples would be the same individual. This was shown by samples from the wet and the dry stand being part of the same individual. The stands were in close enough proximity that the roots spread from one of the stands to the other (Table 3, Individual 3). This individual had four samples from the dry stand, and two samples from the wet stand within the individual